Line Sorter
Compute stock and solvent volumes from target concentration and final volume. Serial dilution tables included.
This tool calculates dilution volumes only, not experimental procedures.
Vstupy
No unit conversion for concentration. Enter C1 and C2 in the same unit.
Rounding & Pipette Limits
Suggest shows candidate volumes and errors; force recalculates with rounded volumes.
Results (Live)
Results update as you typeVarování
Rounding suggestions
| Krok | Factor | Transfer | Solvent | Final | Concentration (relative) | Cumulative |
|---|
Formulas & Terms
Terms
- C1: stock concentration
- C2: target concentration
- V1: volume taken from stock
- V2: final volume
- Fi: step factor (1:Fi)
Equations
- Single: V1 = (C2 × V2) / C1
- Solvent = V2 - V1
- Serial: C_i = C_(i-1) / Fi
- Intermediate: Transfer = V_step / Fi
- Final: Transfer_last = V2 / F_last
Poznámky
- No concentration unit conversion (C1 and C2 must match).
- If C2 mismatch appears, verify the product of all factors.
Časté dotazy
I want µL units.
Switch freely between µL, mL, and L. Volumes are converted automatically.
I get fractional volumes.
Set minimum pipette volume or rounding unit to get realistic candidates and concentration error.
Local presets?
Save frequent factor sets (e.g., 10x×3) locally in your browser.
Can it convert concentration units (mM→µM)?
This tool assumes C1 and C2 are in the same unit. Convert first, then enter values.
My C2 mismatch warning shows up.
The product of step factors may not match the total dilution factor. Check cumulative factor and reached concentration.
Důležité
- This page calculates dilution volumes only and does not provide experimental procedures.
- Follow your institution's SOPs for safety and reproducibility (mixing order, contamination, temperature, handling).
- Validate inputs (units, concentration, viscosity, pipette accuracy) yourself.